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in fusion cloning strategy  (TaKaRa)


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    Structured Review

    TaKaRa in fusion cloning strategy
    In Fusion Cloning Strategy, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/in+fusion+strategy/In-Fusion+HD+Cloning+Plus/bio_rxiv__64898__2026__03__30__715387-228-18-21
    Average 99 stars, based on 1541 article reviews
    in fusion cloning strategy - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. To overexpress Zfp260, the pCDH- Zfp260 -V5 lentivirus expression vector was constructed by cloning and fusing the CDS of mus musculus Zfp260 into the MCS site (EcoRI and BamHI) of the pCDH-V5-Puro plasmid via the In-Fusion strategy (639650, Clontech). .. For lentivirus packaging, a three-plasmid system (pol/gag, VSV-G and pCDH-V5/pCDH- Zfp260 -V5) were transfected into the 293 T cell line (H4-1401, Cyagen Biosciences).

    Construct:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. To overexpress Zfp260, the pCDH- Zfp260 -V5 lentivirus expression vector was constructed by cloning and fusing the CDS of mus musculus Zfp260 into the MCS site (EcoRI and BamHI) of the pCDH-V5-Puro plasmid via the In-Fusion strategy (639650, Clontech). .. For lentivirus packaging, a three-plasmid system (pol/gag, VSV-G and pCDH-V5/pCDH- Zfp260 -V5) were transfected into the 293 T cell line (H4-1401, Cyagen Biosciences).

    Cloning:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. To overexpress Zfp260, the pCDH- Zfp260 -V5 lentivirus expression vector was constructed by cloning and fusing the CDS of mus musculus Zfp260 into the MCS site (EcoRI and BamHI) of the pCDH-V5-Puro plasmid via the In-Fusion strategy (639650, Clontech). .. For lentivirus packaging, a three-plasmid system (pol/gag, VSV-G and pCDH-V5/pCDH- Zfp260 -V5) were transfected into the 293 T cell line (H4-1401, Cyagen Biosciences).

    Article Title: Factor H–Related Protein 1 Drives Disease Susceptibility and Prognosis in C3 Glomerulopathy
    Article Snippet: .. To generate recombinant FHR-1 wild-type protein (rFHR1), a pEZ-M02 vector containing the CFHR1 cDNA was obtained from GeneCopoeia (catalog number EX-Z0243M02) and the cDNA was cloned into a modified version of pCAGGS plasmid21 using an In-Fusion strategy (In-Fusion HD Cloning; Takara Bio USA). .. The recombinant mutant FHR-1 allele with the internal duplication (rFHR-1mut) and a monomeric version of FHR-1 (rFHR-1mon) were also generated using an In-Fusion strategy. rFHR-1mon was generated by mutating three key amino acids within the dimerization motif (Tyr34Ser, Ser36Tyr, and Tyr39Glu), using the same strategy described to generate a monomeric FHR-5.11 The recombinant proteins were transiently expressed in FreeStyle 293-F cells following the manufacturer’s instructions (Life Technologies).

    Plasmid Preparation:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. To overexpress Zfp260, the pCDH- Zfp260 -V5 lentivirus expression vector was constructed by cloning and fusing the CDS of mus musculus Zfp260 into the MCS site (EcoRI and BamHI) of the pCDH-V5-Puro plasmid via the In-Fusion strategy (639650, Clontech). .. For lentivirus packaging, a three-plasmid system (pol/gag, VSV-G and pCDH-V5/pCDH- Zfp260 -V5) were transfected into the 293 T cell line (H4-1401, Cyagen Biosciences).

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). .. GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Sequencing:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. Using high-fidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). .. GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: Using highfidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. .. Using highfidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. ..

    Clone Assay:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. Using high-fidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells
    Article Snippet: .. The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). .. GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Article Title: Factor H–Related Protein 1 Drives Disease Susceptibility and Prognosis in C3 Glomerulopathy
    Article Snippet: .. To generate recombinant FHR-1 wild-type protein (rFHR1), a pEZ-M02 vector containing the CFHR1 cDNA was obtained from GeneCopoeia (catalog number EX-Z0243M02) and the cDNA was cloned into a modified version of pCAGGS plasmid21 using an In-Fusion strategy (In-Fusion HD Cloning; Takara Bio USA). .. The recombinant mutant FHR-1 allele with the internal duplication (rFHR-1mut) and a monomeric version of FHR-1 (rFHR-1mon) were also generated using an In-Fusion strategy. rFHR-1mon was generated by mutating three key amino acids within the dimerization motif (Tyr34Ser, Ser36Tyr, and Tyr39Glu), using the same strategy described to generate a monomeric FHR-5.11 The recombinant proteins were transiently expressed in FreeStyle 293-F cells following the manufacturer’s instructions (Life Technologies).

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: Using highfidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. .. Using highfidelity enzyme (P510, Vazyme), the promoter sequence of the mouse Ctsk gene (TSS + 2 kb) was cloned into the site via In-Fusion strategy (639650, Clontech), resulting in the pAAV-Ctsk-MCS-IRES-ZsGreen vector. ..

    other:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: For lentivirus packaging, a three-plasmid system (pol/gag, VSV-G and pCDH-V5/pCDH-Zfp260-V5) were transfected into the 293 T cell line (H4-1401, Cyagen Biosciences).

    Mass Spectrometry:

    Article Title: Zfp260 choreographs the early stage osteo-lineage commitment of skeletal stem cells.
    Article Snippet: GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). .. GST pull-down, mass spectrometry and analysis The coding sequence of mus musculus Zfp260 was cloned and fused into the pGEX6p-1-GST plasmid using the In-Fusion strategy (639650, Clontech). ..

    Recombinant:

    Article Title: Factor H–Related Protein 1 Drives Disease Susceptibility and Prognosis in C3 Glomerulopathy
    Article Snippet: .. To generate recombinant FHR-1 wild-type protein (rFHR1), a pEZ-M02 vector containing the CFHR1 cDNA was obtained from GeneCopoeia (catalog number EX-Z0243M02) and the cDNA was cloned into a modified version of pCAGGS plasmid21 using an In-Fusion strategy (In-Fusion HD Cloning; Takara Bio USA). .. The recombinant mutant FHR-1 allele with the internal duplication (rFHR-1mut) and a monomeric version of FHR-1 (rFHR-1mon) were also generated using an In-Fusion strategy. rFHR-1mon was generated by mutating three key amino acids within the dimerization motif (Tyr34Ser, Ser36Tyr, and Tyr39Glu), using the same strategy described to generate a monomeric FHR-5.11 The recombinant proteins were transiently expressed in FreeStyle 293-F cells following the manufacturer’s instructions (Life Technologies).

    Modification:

    Article Title: Factor H–Related Protein 1 Drives Disease Susceptibility and Prognosis in C3 Glomerulopathy
    Article Snippet: .. To generate recombinant FHR-1 wild-type protein (rFHR1), a pEZ-M02 vector containing the CFHR1 cDNA was obtained from GeneCopoeia (catalog number EX-Z0243M02) and the cDNA was cloned into a modified version of pCAGGS plasmid21 using an In-Fusion strategy (In-Fusion HD Cloning; Takara Bio USA). .. The recombinant mutant FHR-1 allele with the internal duplication (rFHR-1mut) and a monomeric version of FHR-1 (rFHR-1mon) were also generated using an In-Fusion strategy. rFHR-1mon was generated by mutating three key amino acids within the dimerization motif (Tyr34Ser, Ser36Tyr, and Tyr39Glu), using the same strategy described to generate a monomeric FHR-5.11 The recombinant proteins were transiently expressed in FreeStyle 293-F cells following the manufacturer’s instructions (Life Technologies).



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